Journal
/
/
Enrichissement de la membrane de Bruch des yeux de donneur humain
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Enrichment of Bruch’s Membrane from Human Donor Eyes
DOI:

10:22 min

November 15, 2015

, , ,

Chapitres

  • 00:05Titre
  • 01:14Enrichment of Whole Bruch’s Membrane
  • 05:00Solubilization of Whole Enriched Bruch’s Membrane and Western Blot Analysis
  • 06:34Enrichment of Macular Bruch’s Membrane
  • 08:51Results: Histology and Protein Content of Bruch’s Membrane
  • 09:35Conclusion

Summary

Traduction automatique

Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.

Vidéos Connexes

Read Article