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Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
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Chapitres
- 00:05Titre
- 00:40Creating the Scratch
- 02:51Taking Initial Images of the Scratch
- 04:34Analyzing Initial Scratch Images
- 06:07Taking Final Images of the Scratch and Analyzing Final Scratch Images
- 06:52Results: Migratory of Murine Lung MSCs is Altered After Damage with Cigarette Smoke Extract
- 08:02Conclusion
The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.
