/
/
Sekventiell Utvinning av lösliga och olösliga alfa-synuklein från Parkinson Brains
This content is Free Access.
Chapitres
- 00:05Titre
- 00:36Brain Sample Homogenization and Differential Ultracentrifugation
- 04:37Immunoblotting of Samples
- 07:25Results: Distinct Species of Alpha-Synuclein Detected by Western Immunoblot
- 08:35Conclusion
Here, we present a protocol for the isolation of increasingly insoluble/aggregated alpha-synuclein (α-syn) from post-mortem human brain tissue. Through the utilization of buffers with increasing detergent strength and high-speed ultracentrifugation techniques, the variable properties of α-syn aggregation in diseased and non-diseased tissue can be examined.
