This content is Free Access.
Chapitres
- 00:05Titre
- 00:59Bone Marrow-derived Macrophage Polarization
- 02:17Extracellular Flux Assay Preparation
- 03:46Bioenergetic Characterization of Polarized M1 and M2 Macrophages
- 05:17Results: Metabolic Characteristics of Control M0 and M1- and M2-polarized Macrophages
- 06:49Conclusion
Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.
