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Verbeterde Generatie van geïnduceerde Cardiomyocytes Met behulp van een polycistronisch Construct uitdrukken optimale verhouding van GATA4, MEF2C en Tbx5
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Chapitres
- 00:05Titre
- 00:57Generation of Neonatal Mouse Cardiac Fibroblasts via Explant Culture
- 03:20Generation of Neonatal Mouse Cardiac Fibroblasts via Enzymatic Digestion
- 05:02Isolation of Thy1.2+ Fibroblasts by Magnetic-activated Cell Sorting (MACS)
- 07:17Retroviral Mediated Reprogramming of Isolated Cardiac Fibroblasts
- 08:52Results: Retroviral Reprogramming of Cardiac Fibroblasts to Induced Cardiac Myocytes
- 09:38Conclusion
We describe here a protocol for the generation of iCMs using retrovirus-mediated delivery of Gata4, Tbx5 and Mef2c in a polycistronic construct. This protocol yields a relatively homogeneous population of reprogrammed cells with improved efficiency and quality and is valuable for future studies of iCM reprogramming.
