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Bio-ingénierie
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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

DOI:
10.3791/53431-v

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08:28 min

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May 26, 2016

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, , , , , , ,
1Department of Chemical Engineering and Centre for Regenerative Medicine,University of Bath, 2MRC Centre for Drug Safety Science and Institute of Translational Medicine,University of Liverpool, 3Mechanical Engineering,University College London, 4Department of Applied Mathematics,Liverpool John Moores University

Chapitres

  • 00:05Titre
  • 00:35Module Fabrication
  • 01:58System Setup and Sterilization
  • 04:12Seeding the Module
  • 06:01Excision of Fibers
  • 06:44Results: Seeding, Proliferation, Viability and Biochemical Analysis
  • 08:06Conclusion

Summary

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Traduction automatique

The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.

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Hollow Fiber BioreactorIn Vitro ModelLiver Cell FunctionPhysiologically Relevant EnvironmentSinusoidBile CanaliculiCell CultureDrug DosingBorosilicate GlassSiliconizingAutoclaveSilicone GluePTFE TapeEthanol SterilizationPump TubingModule ConnectorsPermeate LineRetentate Line

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