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Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli
 

Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli

Article DOI: 10.3791/53432-v • 09:43 min • December 19th, 2015
December 19th, 2015 •
1, 1, 1, 1
1Virologie et Immunologie Moléculaires, INRA

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A two-step chromatographic method is described for the purification of recombinant Shadoo protein expressed as inclusion bodies in Escherichia coli, as well as a protocol to fibrillate purified Shadoo into amyloid structures.

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Purification Refolding Amyloid Fibrils (His)6-tagged Recombinant Shadoo Protein Inclusion Bodies E. Coli Chromatographic Method Solubilized Urea Ni2+-charged Column Ion Affinity Chromatography Imidazole Gradient Size Exclusion Chromatography Highly Purified Protein Desalted Salts Biophysical Studies Biochemical Studies Protein Conformation Disorders Prion Diseases Neurodegenerative Diseases Deposition Stable Amyloid Fibrils
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