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Imaging subzellulärer Strukturen in der Living Zebrafischembryo
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1Institute of Neuronal Cell Biology,Technische Universität München, 2Cell Biology, Department of Biology, Faculty of Science,Utrecht University, 3Faculty of Biology,Ludwig-Maximilians-Universität-München, 4Adolf-Butenandt-Institute, Biochemistry,Ludwig-Maximilians-Universität-München, 5German Center for Neurodegenerative Diseases, 6Laboratory of Brain Development and Repair,The Rockefeller University
Chapitres
- 00:05Titre
- 00:50Prepare Embryos for Imaging on an Upright Microscope
- 02:37Wide-Field Microscopy
- 04:43Confocal Microscopy
- 08:47Results: Wide-field and Confocal Imaging of the Dynamics of Mitochondria and Centrosomes in Zebrafish
- 10:28Conclusion
Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.
