Journal
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Immunologie et infection
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Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

DOI:
10.3791/53460-v

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09:13 min

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January 13, 2016

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, , ,
1Department of Biological Sciences,Carnegie Mellon University, 2Infectious Diseases,Harbor-UCLA Medical Center

Chapitres

  • 00:05Titre
  • 00:59Preparation of Reagents and Instruments
  • 01:47RNA Extraction from Infected Tissues
  • 04:01Gene Expression Profiling Using Digital Bar-coding
  • 06:55Results: C. albicans Gene Expression Using Digital Bar-coding
  • 08:21Conclusion

Summary

Traduction automatique

Traduction automatique

We developed a protocol that is fast, sensitive and reproducible for pathogen gene expression profiling during an infection.

Tags

Gene Expression ProfilingDigital Bar-codingPathogen Gene ExpressionInfectious DiseaseC. AlbicansRNA ExtractionPhenol-chloroform Extraction

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