Journal
/
Biologie
/
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

DOI:
10.3791/53470-v

•

09:08 min

•

December 16, 2015

•

, ,
1Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 2School of Biological and Chemical Sciences,Queen Mary University of London

Chapitres

  • 00:05Titre
  • 01:00Chromosome Preparation
  • 05:25Fluorescent In Situ Hybridization (FISH)
  • 07:11Results: FISH Performed on Mitotic Metaphase Chromosome Spreads of Hordeum vulgare, H. Bulbosum, and Secale cereale Prepared by the Aid-dry Dropping Method
  • 08:25Conclusion

Summary

Traduction automatique

Traduction automatique

A protocol is described for the preparation of high-quality mitotic plant chromosome spreads by a fast air-dry dropping method suitable for the FISH detection of single and high copy DNA probes.

Tags

Chromosome Spread PreparationFISHAir-dry Drop MethodPlant CytogeneticsHordeumSecale

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/53470?article_type=v

Pyrosequencing: A Simple Method for Accurate Genotyping
check_url/fr/53470?article_type=v

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
check_url/fr/53470?article_type=v

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
check_url/fr/53470?article_type=v

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
check_url/fr/53470?article_type=v

Chromosome Preparation From Cultured Cells
check_url/fr/53470?article_type=v

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras
check_url/fr/53470?article_type=v

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
check_url/fr/53470?article_type=v

Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture
check_url/fr/53470?article_type=v

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
check_url/fr/53470?article_type=v

Standardizing a Non-Lethal Method for Characterizing the Reproductive Status and Larval Development of Freshwater Mussels (Bivalvia: Unionida)

Read Article