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Un ensayo de conducta para Mechanosensation de clones en MARCM-basa<em> Drosophila melanogaster</em
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
DOI:

05:48 min

December 30, 2015

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Chapitres

  • 00:05Titre
  • 00:48MARCM Fly Generation and Timed Egg-Laying
  • 02:11Heat Shock Induction
  • 02:54Behavioral Testing
  • 04:38Results: Lack of Response in Mechanosenstivive Mutants
  • 05:10Conclusion

Summary

Traduction automatique

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

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