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High-Resolution Kvantitativ immunoguld Analyse af membranreceptorer på retinale Ribbon synapser
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Neurosciences
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Journal JoVE Neurosciences
High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

High-Resolution Kvantitativ immunoguld Analyse af membranreceptorer på retinale Ribbon synapser

DOI:
10.3791/53547-v

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09:24 min

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February 18, 2016

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, , ,
1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 2Advanced Imaging Core, National Institute on Deafness and Other Communication Disorders,National Institutes of Health

Chapitres

  • 00:05Titre
  • 00:49Retina Tissue Fixation
  • 01:48Freeze-substitution
  • 04:35Postembedding EM Immunogold Immunocytochemistry
  • 06:42Quantification
  • 07:43Results: Immunogold Labeling Showing Synaptic and Perisynaptic Localization of Glutamate Receptors at RGC Dendrites Labeled by CTB
  • 08:45Conclusion

Summary

Traduction automatique

Traduction automatique

The postembedding immunogold method is one of the most effective ways to provide high-resolution analyses of the subcellular localization of specific molecules. Here we describe a protocol to quantitatively analyze glutamate receptors at retinal ribbon synapses.

Tags

Keywords: Quantitative Immunogold AnalysisMembrane ReceptorsRetinal Ribbon SynapsesFreeze-substitutionUltra-thin SectioningRetinal NeurobiologyProtein LocalizationProtein DensityRetinal Circuit

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