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Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
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Médecine
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Journal JoVE Médecine
Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

DOI:
10.3791/53559-v

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07:07 min

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February 21, 2016

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1Cell and Matrix Biology, Institute of Zoology,Johannes Gutenberg-Universität Mainz

Chapitres

  • 00:05Titre
  • 00:39Cochlea Dissection
  • 03:15Post Immunohistochemistry Mounting and Imaging
  • 04:39Results: Morphology and Immunofluorescence in Control and Cilia Mutant Cochlea
  • 06:16Conclusion

Summary

Traduction automatique

Traduction automatique

Primary cilia influence various signaling pathways. The mammalian cochlea is ideal for examining planar cell polarity (PCP) signaling. Cilia dysfunction affects cochlear outgrowth, cellular patterning and hair cell orientation, readouts of PCP. Our goal is to analyze PCP signaling in mouse cochlea via phenotypic analysis, immunohistochemistry and scanning electron microscopy.

Tags

Planar-cell-polarityCiliary MutantCochleaInner EarTectorial MembraneOrgan Of CortiImmunohistochemistryScanning Electron Microscopy

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