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Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
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Journal JoVE Médecine
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

DOI:
10.3791/53579-v

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09:30 min

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March 04, 2016

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, , , , , , , , , ,
1Burns Centre and Emilia Romagna Regional Skin Bank, 2Human Brain Wave srl, 3Plastic and Reconstructive Surgery,AOU “Ospedali Riuniti”, 4Department of Public Health, Experimental Medicine, Anatomy and Forensic,University of Pavia, 5C.H.T Centre for Health Technologies,University of Pavia

Chapitres

  • 00:05Titre
  • 00:45Bio-complex Building for Clinical Application
  • 02:59Collection Disaggregation and In Vitro Analysis of Tissues
  • 06:40Results: Skin Disaggregation and Tissue Characterization
  • 08:18Conclusion

Summary

Traduction automatique

Traduction automatique

The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.

Tags

Skin Micro-graftsAutologous Skin GraftsWound HealingRegenerative MedicineChronic WoundsBurnsPost-traumatic UlcersCollagen SpongesBiocomplexDisaggregation MethodHistologyHematoxylin-eosin StainingLight Microscopy

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