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Meiotiske Spindel Vurdering i museoocytter af siRNA-medieret Silencing
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Biologie
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Journal JoVE Biologie
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotiske Spindel Vurdering i museoocytter af siRNA-medieret Silencing

DOI:
10.3791/53586-v

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09:16 min

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October 11, 2015

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1Department of Physiology and Pharmacology,College of Veterinary Medicine, University of Georgia

Chapitres

  • 00:05Titre
  • 00:54Collecting Mouse Oocytes
  • 02:08Oocyte Microinjection
  • 04:42Culturing and Staining the Injected Oocytes
  • 07:52Results: Disruption of Pericentrin Expression in Oocytes during Meiotic Division
  • 08:37Conclusion

Summary

Traduction automatique

Traduction automatique

Her præsenterer vi en protokol for specifik siRNA-medieret mRNA udtømning efterfulgt af immunofluorescensanalyse at evaluere meiotisk spindel samling og organisation i museoocytter. Denne protokol er velegnet til in vitro-udtømning af udskrifter og funktionel vurdering af forskellige spindel og / eller MTOC-associerede faktorer i oocytter.

Tags

Meiotic SpindleSiRNAChromosome SegregationAneuploidyMicrotubule Spindle ApparatusMTOCMouse OocytesImmunofluorescence Analysis

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