Journal
/
Biologie
/
在c-fos蛋白免疫组织学检测：一个有用​​的工具，中央通路在特定的生理反应所涉及的标记<em>在体内</em>和<em>离体</em
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
Please note that all translations are automatically generated. Click here for the English version.

在c-fos蛋白免疫组织学检测：一个有用​​的工具，中央通路在特定的生理反应所涉及的标记<em>在体内</em>和<em>离体</em

DOI:
10.3791/53613-v

•

05:44 min

•

April 25, 2016

•

, , , , , ,
1Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363,University Paris 13, 2UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique,Sorbonne Universités, 3Laboratory of Excellence GR-Ex, 4Laboratory MOVE (EA 6314),University of Poitiers

Chapitres

  • 00:05Titre
  • 00:44Day 1 Endogenous Peroxidase Activity Destruction, Non-specific Binding Site Blockade and Incubation with the Primary Antibody
  • 01:44Day 3 Incubation with Secondary Antibody and Development of a Color Reaction
  • 03:21Sample Mounting
  • 04:16Results: c-FOS-positive Neurons on Sections of the Medulla Oblongata in Control Mice and Mice Submitted to Hypoxia In Vivo
  • 05:17Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.

Tags

C-FOS ProteinImmunohistological DetectionMarkerCentral PathwaysPhysiological ResponsesIn VivoEx VivoNeuronal ActivityBrain MappingBrain RegionsNeurophysiological FieldC-FOS GeneStimulationQuantificationTest SimulationCoronal Brain Stem SectionsWashPBSEndogenous Peroxidase ActivityHydrogen PeroxideBlock Nonspecific Binding SitesGoat SerumPBSTPolyclonal Antibody

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/53613?article_type=v

在体内的单细胞电穿孔内完整的大脑发育
check_url/fr/53613?article_type=v

文化早期鸡胚体外（新文化）的方法
check_url/fr/53613?article_type=v

在子宫内和前体内的基因表达在小鼠视网膜神经节细胞的电穿孔
check_url/fr/53613?article_type=v

微图案的表面上的蛋白质-蛋白质相互作用的体内检测
check_url/fr/53613?article_type=v

作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列
check_url/fr/53613?article_type=v

一种新型的<em>体外</em小鼠胚胎心脏的培养方法
check_url/fr/53613?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/fr/53613?article_type=v

染色质免疫沉淀拟南芥蛋白-DNA相互作用的鉴定<em>在体内</em
check_url/fr/53613?article_type=v

测量的刚度<em>离体</em>鼠标主动脉用原子力显微镜
check_url/fr/53613?article_type=v

在体内人细胞 Rb 蛋白 sumo 的检测与分析

Read Article