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Chapitres
- 00:05Titre
- 00:49Electroporation of Fibroblasts with EYFP-Parkin and pDsRed2-Mito Expression Vectors
- 03:44Time-lapse Video Microscopy
- 07:20Results: EYFP-Parkin Aggregates in Mitochondrial Membranes During Mitophagy
- 08:55Conclusion
Herein, we describe in detail a time-lapse video microscopy approach to measuring the temporal recruitment of EYFP-Parkin during the selective removal of damaged mitochondria. This dynamic process of EYFP-Parkin-dependent removal of damaged mitochondria can be used as an indicator of cellular health under different experimental conditions.
