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Chapitres
- 00:05Titre
- 00:55Extraction of Nucleic Acids
- 02:03Amplification Procedure
- 03:57Limit of Detection of qRT-PCR
- 05:54Limit of Detection of the Method Determined by Two Independent Trials
- 07:04Results: Validation of Quantitative PCR Method for Equid Herpesvirus-2 Diagnosis
- 08:45Conclusion
Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.
