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JoVE Journal Neuroscience
Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique
 

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

Article DOI: 10.3791/53673-v • 09:54 min • February 26th, 2016
February 26th, 2016 •
1,2, 1, 1,2
1Brain Structure and Function Group, Neuroscience Research Australia, 2School of Medical Sciences, The University of New South Wales

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Automatic Translation

Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.

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Keywords: Serotonergic Fibers Mouse Spinal Cord CLARITY/CUBIC Technique 3D Neuroanatomy Histology Central Nervous System Cardiovascular System Tissue Clearing Hydrogel Perfusion Tissue Harvesting Spinal Cord Dissection
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