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Isolement de CD 90+ fibroblastes / myofibroblastes de spécimens humains congelés gastro-intestinaux
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Chapitres
- 00:05Titre
- 00:39Enzymatic Tissue Digestion
- 03:46Results: Frozen GI Tissue Primary MF Culture Generation
- 04:51Conclusion
Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.
