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HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
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Neurosciences
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Journal JoVE Neurosciences
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

DOI:
10.3791/53717-v

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07:30 min

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July 09, 2016

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, ,
1Department of Psychiatry,Icahn School of Medicine at Mount Sinai, 2Department of Neurology,Icahn School of Medicine at Mount Sinai, 3Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 4Department of Physiology and Biophysics,Virginia Commonwealth University Medical School

Chapitres

  • 00:05Titre
  • 00:56Stereotaxic Surgery to Introduce HSV Chimeric Constructs
  • 03:32Head Twitch Response Experiment
  • 05:04Data Review
  • 05:33Résultats
  • 06:35Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes how to inject viral vectors into the mouse frontal cortex to test behavioral assays that require GPCR heteromeric formation.

Tags

HSVTransgene ExpressionGPCR HeteromersMouse ModelBehavioral PhenotypesSerotonin 2aMetabotropic Glutamate Receptor 2Stereotactic SurgeryVirus-mediated Gene TransferAnesthesiaInjectionFrontal Cortex

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