Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg, 2Kidney Control of Homeostasis,Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg
Chapitres
- 00:05Titre
- 00:52Preparing the Organ Bath System
- 02:01Isolation of Mouse Aortas
- 03:03DHE and DAF-2DA Staining
- 04:47En Face Mounting and Confocal Microscopic Imaging
- 06:42Results: eNOS-uncoupling in Obese Mice
- 07:56Conclusion
Bu yazıda floresan boya tr yüz eşzamanlı algılama ve hücre içi nitrik oksit görselleştirme (NO) ve süperoksit anyon (O 2 diaminofluorescein-2 diasetat (DAF-2DA) ve dihydroethidium (DHE) kullanarak adapte nispeten kolay bir yöntem tarif .- ) sırasıyla, taze bir obezite fare modelinin sağlam aorta izole edilmiştir.
