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Assessing Specificity of Anticancer Drugs In Vitro
Journal JoVE
Médecine
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Journal JoVE Médecine
Assessing Specificity of Anticancer Drugs In Vitro

Assessing Specificity of Anticancer Drugs In Vitro

DOI:
10.3791/53752-v

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09:39 min

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March 23, 2016

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1Department of Maxillofacial Surgery,University Medical Center Hamburg-Eppendorf, 2Department of Neurology,University Medical Center Hamburg-Eppendorf, 3Laboratory for Tumor Biology and Regenerative Medicine,University Medical Center Hamburg-Eppendorf

Chapitres

  • 00:05Titre
  • 01:00Mix Culture of Both Tumor Cells and Non-tumor Cells and Drug Treatment
  • 02:44Preparing DNA for Digital PCR
  • 04:51Digital PCR
  • 06:22Calculating Tumor Cell Proportion
  • 07:20Results: Dose-Dependent Change of Tumor Cell Proportion and Responses of Tumor/Non-Tumor Cells
  • 08:14Conclusion

Summary

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The goal of this protocol is to assess specificity of anticancer drugs in vitro using mixed cultures containing both tumor and non-tumor cells.

Tags

Keywords: In VitroAnticancer DrugsTumor CellsNon-tumor CellsSpecificityDrug DiscoveryPersonalized Cancer CareCell CultureNilotinibDose-dependentHotSHOT MethodCell LysisPCRDNA

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