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An Improved Method for Rapid Intubation of the Trachea in Mice
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Médecine
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Journal JoVE Médecine
An Improved Method for Rapid Intubation of the Trachea in Mice

An Improved Method for Rapid Intubation of the Trachea in Mice

DOI:
10.3791/53771-v

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08:46 min

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February 22, 2016

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, ,
1Department of Environmental and Occupational Health Sciences,University of Washington, 2Department of Medicine,Cedars-Sinai Medical Center, 3Division of Pulmonary and Critical Care Medicine,University of Washington

Chapitres

  • 00:05Titre
  • 00:45Preparations
  • 03:22Intubation
  • 07:11Results: Weight Loss and Barrier Dysfunction Following Administration of Bleomycin
  • 08:15Conclusion

Summary

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This article presents a rapid and simple method for administering bleomycin directly into the mouse trachea via intubation. Key advantages of this method are that it is highly reproducible, easy to master, and does not require specialized equipment or lengthy recovery times.

Tags

Keywords: IntubationTracheaMouseLungAdministrationReagentsAcute Lung InjuryFibrogenesisSterilizedProcedure BoardSpirometerIsofluraneAnesthesia

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