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A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
DOI:

11:19 min

March 05, 2016

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Chapitres

  • 00:05Titre
  • 00:5924-well Plate Preparation for High and Low Density Neuron Culture
  • 01:48Brain Removal and Hippocampi Dissection of E16.5 – E17.5 Mouse Embryos
  • 04:02Enzymatic Digestion and Separation into Single Neurons
  • 06:53Plating of Neurons and Long Term Co-Culture
  • 08:51Results: Experimental Manipulations of Low Density Neurons Grown in Co-Culture
  • 10:23Conclusion

Summary

Traduction automatique

Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.

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