Journal
/
Biologie
/
Microscopia di lievito di fissione del ciclo di vita sessuale
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Microscopy of Fission Yeast Sexual Lifecycle
Please note that all translations are automatically generated. Click here for the English version.

Microscopia di lievito di fissione del ciclo di vita sessuale

DOI:
10.3791/53801-v

•

07:47 min

•

March 09, 2016

•

, , , ,
1Department of Fundamental Microbiology,University of Lausanne

Chapitres

  • 00:05Titre
  • 01:00Culturing Fission Yeast Strains for Mating Experiments
  • 03:31Preparing Agarose Pads and Mounting Cells for Imaging
  • 05:52Long-term Monitoring of the Reproductive Process in Fission Yeast
  • 07:20Conclusion

Summary

Traduction automatique

Traduction automatique

We provide a reproducible basic method for the long-term microscopy of the fission yeast sexual lifecycle. With minor adjustments described, the presented protocol allows research focus on different steps of the reproductive process.

Tags

Keywords: Fission YeastSexual LifecycleTime-lapse ImagingMatingPolarized GrowthCell-cell FusionMeiosisSporulationHomothallicHeterothallicMating Type SwitchingMinimum Sporulation MediumOptical DensityCell Culture

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/53801?article_type=v

Purificazione dei mitocondri da cellule di lievito
check_url/fr/53801?article_type=v

Lievito Colony metodo di inserimento
check_url/fr/53801?article_type=v

Un saggio microscopia a fluorescenza per Mitophagy monitoraggio nel lievito Saccharomyces cerevisiae
check_url/fr/53801?article_type=v

High-throughput iperespressione schermo lievito plasmidi
check_url/fr/53801?article_type=v

Quantitativa vivo cellulare-microscopio a fluorescenza Analisi di lievito Fission
check_url/fr/53801?article_type=v

Sviluppo sessuale e scarico in ascospore Fusarium graminearum</em
check_url/fr/53801?article_type=v

Diffondere superfici e Immunostaining di cromosomi del lievito
check_url/fr/53801?article_type=v

Analisi spazio-temporale di Cytokinetic Eventi in lievito di fissione
check_url/fr/53801?article_type=v

Microscopia 4D del lievito
check_url/fr/53801?article_type=v

Preparazione del campione biologico di congelamento ad alta pressione, aumento di contrasto assistita da microonde e Minimal resina incorporamento per Imaging con volumi

Read Article