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Lo sviluppo del liofilizzato Loop-mediate isotermici amplificazione reagenti per la rilevazione di<em> Burnetii Coxiella</em
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
DOI:

07:27 min

April 18, 2016

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Chapitres

  • 00:05Titre
  • 00:40Prepare Plasmid DNA Dilutions as Standard for LAMP Reaction
  • 01:24Perform Standard LAMP Reaction
  • 02:54Perform LAMP Reaction with Reconstituted Reagents
  • 05:17Results: Detection of Coxiella burnetii with the LAMP Reaction
  • 06:37Conclusion

Summary

Traduction automatique

We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.

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