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High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
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Journal JoVE Biologie
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

DOI:
10.3791/53843-v

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12:59 min

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April 30, 2016

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,
1Boyce Thompson Institute for Plant Research, 2Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science,Cornell University

Chapitres

  • 00:05Titre
  • 00:36CRISPR Vector Construction
  • 06:36Hairy Root Transformation
  • 10:59Results: Hairy Root Cultures and Detection of DNA Modification
  • 12:17Conclusion

Summary

Traduction automatique

Traduction automatique

Using DNA assembly, multiple CRISPR vectors can be constructed in parallel in a single cloning reaction, making the construction of large numbers of CRISPR vectors a simple task. Tomato hairy roots are an excellent model system to validate CRISPR vectors and generate mutant materials.

Tags

CRISPRVector ConstructionDNA ModificationsTomato Hairy RootsFunctional GenomicsCloning ProcedureGuide RNAKnockout MutantsPCR AmplificationHigh-ThroughputDNA Assembly

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