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Chapitres
- 00:05Titre
- 01:13Preparation of Electro-competent Bacterial Cells for Transformation
- 02:33Electroporation of Bacterial Cells with Complementation Plasmids
- 03:39Transformation of AOH1 to Facilitate Functional Complementation Using L,L-diaminopimelate Aminotransferase
- 04:18Transformation of TKL-11 to Facilitate Functional Complementation Using UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate Ligase
- 05:12Transformation of Mutant Hx699 to Facilitate Functional Complementation of Hypomucoid Phenotype of Novosphingobium sp. Strain Hx699
- 06:02Results: Functional Complementation Analysis
- 07:44Conclusion
Valideringen af enzymatiske aktiviteter i forbindelse med biokemiske veje kan belyses ved hjælp af funktionel komplementering analyse (FCA). Beskrevet i dette manuskript er FCA-analysen viser den enzymatiske aktivitet af enzymer involveret i metabolismen af aminosyrer, bakteriel strenge respons og bakteriel peptidoglycan biosyntese.
