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Kombinierte optogenetische und Gefrierbruch Replica Immunmarkierung Eingang spezifische Anordnung der Glutamate Receptors in der Maus Amygdala zu untersuchen
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Chapitres
- 00:05Titre
- 01:30Specimen Preparation
- 02:22High Pressure Freezing
- 03:49Preparation of the Electron Beam Gun
- 04:42Freeze-fracture and Replication
- 06:26Immunolabeling and Analysis
- 07:18Results: AMPA-Rs and NMDA-Rs Immunolabeling at Identified PIN/MGn-ITC Synapses
- 08:42Conclusion
This article illustrates how the expression of neurotransmitter receptors can be quantified and the pattern analyzed at synapses with identified pre and postsynaptic elements using a combination of viral transduction of optogenetic tools and the freeze-fracture replica immunolabeling technique.
