Journal
/
Biologie du développement
/
Isolation and Characterization of Single Cells from Zebrafish Embryos
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Isolation and Characterization of Single Cells from Zebrafish Embryos

Isolation and Characterization of Single Cells from Zebrafish Embryos

DOI:
10.3791/53877-v

•

09:25 min

•

March 12, 2016

•

, , , ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2McAllister Heart Institute,University of North Carolina at Chapel Hill, 3Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

Chapitres

  • 00:05Titre
  • 01:16Staged Embryo Acquisition and Single Cell Dissociation
  • 05:03Microfluidic Chip Cell Loading
  • 06:55Results: Representative Single Zebrafish Nkx2.5ZsY Positive Cell Analysis
  • 08:16Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

Tags

Keywords: Single Cell IsolationZebrafish EmbryosMicrofluidic-based Single-cell AnalysisGene ExpressionBiologie du développementCell HeterogeneityCell DissociationCell PreparationChorion RemovalDe-yolkingCell WashingCell Resuspension

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/53877?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/fr/53877?article_type=v

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
check_url/fr/53877?article_type=v

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
check_url/fr/53877?article_type=v

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
check_url/fr/53877?article_type=v

Single-cell Photoconversion in Living Intact Zebrafish
check_url/fr/53877?article_type=v

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
check_url/fr/53877?article_type=v

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
check_url/fr/53877?article_type=v

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
check_url/fr/53877?article_type=v

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
check_url/fr/53877?article_type=v

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Read Article