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TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
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Médecine
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Journal JoVE Médecine
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

DOI:
10.3791/53879-v

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09:18 min

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June 02, 2016

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, , ,
1Research Institute for Healthy Living,Niigata University of Pharmacy and Applied Life Sciences

Chapitres

  • 00:05Titre
  • 00:41Construction of a Heptamer-type sgRNA Library
  • 04:26Screening of the sgRNA Library for Cell Viability
  • 05:44Evaluation of Effective sgRNAs by Flow Cytometry
  • 07:15Effects of Heptamer-type sgRNAs on Leukemia and Myeloma Cell Lines
  • 08:41Conclusion

Summary

Traduction automatique

Traduction automatique

Here we present a protocol for screening of a heptamer-type sgRNA library for potential therapeutic drugs against blood cancers.

Tags

Keywords: TRUE Gene SilencingSmall Guide RNA (sgRNA)Heptamer-type SgRNA LibraryTRNase ZLRNA-directed Gene SilencingCancer Therapeutic AgentsBlood CancersMyelomaLeukemiaCell ViabilityApoptosisFlow Cytometry

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