/
/
Identifikation af kritiske forhold for Immunfarvning i Ærten Bladlus embryoner: Stigende Tissue permeabilitet og aftagende baggrundsfarvning
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:58Culture of Aphids
- 02:06Dissection and Fixation of Ovaries
- 03:02Treatment of Ovaries
- 04:09Antibody Staining
- 05:09Mounting the Aphid Embryos
- 07:00Results: Aphid Ovary Dissection and Whole-mount Immunostaining with Aphid-specific Conditions to Increase Tissue Permeability and Decrease Background Staining
- 08:11Conclusion
A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.
