Journal
/
Recherche en cancérologie
/
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

DOI:
10.3791/53884-v

•

09:25 min

•

October 14, 2016

•

, , , , , , , , ,
1Department of Surgery and Translational Medicine (DCMT),University of Florence, 2Neurofarba Department,University of Florence, 3Department of Traumatology and General Orthopedics,Azienda Ospedaliera Universitaria Careggi

Chapitres

  • 00:05Titre
  • 01:20Establishment of an Osteosarcoma Cell Line and the Sarcosphere Assay
  • 03:12Isolation of Sarcospheres
  • 05:48In Vitro Analysis to Characterize the Osteosarcoma Cancer Stem Cells
  • 06:24Results: Establishing a Verified Culture of Cancer Stem Cells
  • 07:55Conclusion

Summary

Traduction automatique

Traduction automatique

The presence of cancer stem cells (CSCs) in bone sarcomas has recently been linked to their pathogenesis. In this article, we present the isolation of CSCs from primary cell cultures obtained from human biopsies of conventional osteosarcoma (OS) using the ability of CSCs to grow under nonadherent conditions.

Tags

Keywords: Cancer Stem CellsOsteosarcomaSarcosphereIn VitroCell CultureCell IsolationCell SuspensionGrowth FactorsEGFBFGFUltra-low Attachment PlatesHemocytometerPhase Contrast Microscopy

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/53884?article_type=v

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
check_url/fr/53884?article_type=v

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
check_url/fr/53884?article_type=v

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures
check_url/fr/53884?article_type=v

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
check_url/fr/53884?article_type=v

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
check_url/fr/53884?article_type=v

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
check_url/fr/53884?article_type=v

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
check_url/fr/53884?article_type=v

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
check_url/fr/53884?article_type=v

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
check_url/fr/53884?article_type=v

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Read Article