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Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients’ Plasma Through qPCR: A Feasible Liquid Biopsy Tool
Journal JoVE
Médecine
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Journal JoVE Médecine
Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients’ Plasma Through qPCR: A Feasible Liquid Biopsy Tool
DOI:

08:49 min

May 27, 2016

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Chapitres

  • 00:05Titre
  • 00:49Exosome Isolation from Plasma Samples
  • 03:11Reverse Transcription of Exosomal miRNAs
  • 04:25RT-PCR Analysis of Exosomal miRNAs
  • 06:43Results: miRNA Analysis from NSCLC Patient Plasma
  • 07:43Conclusion

Summary

Traduction automatique

This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.

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