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एक विधि को लक्षित और चूहे में अलग-श्वासनली innervating संवेदी न्यूरॉन्स के लिए
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Chapitres
- 00:05Titre
- 00:38Sensory Ganglia Dissociation
- 04:10RNA Extraction
- 04:59Results: Representative FACS and RNA Extraction of Dissociated Ganglia Cells
- 05:51Conclusion
Organ specific sensory neurons are difficult to identify. Fast Blue tracing is used to identify nodose neurons innervating the airways for cell sorting. Sorted nodose neurons are used to extract high quality ribonucleic acid (RNA) for sequencing. Using this protocol, gene expression of airway specific neurons is determined.
