Journal
/
Biologie du développement
/
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

DOI:
10.3791/53921-v

•

10:05 min

•

April 07, 2016

•

, , ,
1Molecular Genetics,Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), 2Faculty of Biology and Pharmacy,Friedrich Schiller University of Jena, 3Carl Zeiss Microscopy GmbH

Chapitres

  • 00:05Titre
  • 01:17Collecting Fertilized Eggs
  • 02:27Microinjecting Developing Embryos
  • 04:21Mounting Developing Embryos for Live Imaging
  • 06:59Compensating for Focal Drift
  • 08:04Results: Normal and Disturbed Kidney Development
  • 09:25Conclusion

Summary

Traduction automatique

Traduction automatique

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.

Tags

ZebrafishKidney DevelopmentTime-lapse ImagingDissecting MicroscopeOptical SectioningBiologie du développementFluorescent MicroscopyAffordable TechniqueModel OrganismsTransgenic GFPEmbryoMicro-injectionMorpholinoMicro-manipulator

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/53921?article_type=v

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
check_url/fr/53921?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/fr/53921?article_type=v

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
check_url/fr/53921?article_type=v

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos
check_url/fr/53921?article_type=v

An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
check_url/fr/53921?article_type=v

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
check_url/fr/53921?article_type=v

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
check_url/fr/53921?article_type=v

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
check_url/fr/53921?article_type=v

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
check_url/fr/53921?article_type=v

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Read Article