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Murine Hind Limb os long Dissection et moelle osseuse Isolation
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
DOI:

07:17 min

April 14, 2016

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Chapitres

  • 00:05Titre
  • 00:41Hind Limb Long Bone Dissection
  • 03:01Long Bone Preparation For Bone Marrow Isolation
  • 04:25Bone Marrow Isolation
  • 05:29Results: Representative Downstream Analyses and Applications of Rapid DIssected Mouse Long Bones and Bone Marrow
  • 06:28Conclusion

Summary

Traduction automatique

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

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