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藻酸盐珠多生物催化剂辅因子再生和可重用性改进的固定化

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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

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藻酸盐珠多生物催化剂辅因子再生和可重用性改进的固定化

DOI:

10.3791/53944-v

•

09:27 min

•

April 22, 2016

•

Hui Gao, Eshita Khera, Jung-Kul Lee, Fei Wen

¹Department of Chemical Engineering,Konkuk University, ²Department of Chemical Engineering,University of Michigan

Chapitres

00:05Titre
00:45Whole-cell Biocatalysts Preparation
02:05Biosynthesis of L-xylulose by Coupling E. coli_HjLAD and E. coli_SpNOx for Cofactor Regeneration
03:51Immobilization of Recombinant Whole-cell Catalysts in Calcium Alginate Beads
06:25Stability Assay of Immobilized Biocatalysts for L-xylulose Production
07:15Results: Co-immobilization of Whole-cell Biocatalysts Improves Reusability
08:55Conclusion

Summary

Traduction automatique

呈现为共固定化全细胞生物催化剂用于辅因子再生和改善的可重用性，使用生产L-木酮糖的作为例子的协议。辅因子再生是通过偶合表达功能上互补酶2 大肠杆菌菌株实现;全细胞生物催化剂固定化是通过细胞封装在藻酸钙珠来实现。

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