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Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates
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Journal JoVE Bio-ingénierie
Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates

Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates

DOI:
10.3791/54004-v

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08:16 min

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May 09, 2016

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, , , ,
1Department of Pathology,University of Alabama at Birmingham, 2Department of Biomedical Engineering,University of Alabama at Birmingham

Chapitres

  • 00:05Titre
  • 00:46Preparation of Cells in Extracellular Matrix
  • 01:36Surrogate Preparation
  • 03:51Surrogate Fixation and Processing
  • 05:27Measuring Cell Density
  • 06:39Results: Representative Images of a H&E-stained Section from Perfused and Solid Surrogates
  • 07:37Conclusion

Summary

Traduction automatique

Traduction automatique

We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.

Tags

Keywords: 3D Cell CultureBreast Cancer SurrogatesExtracellular Matrix (ECM)In VitroCell Growth MeasurementBioreactorCell Viability

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