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Chapitres
- 00:05Titre
- 00:58Preparing the Microchannel Device for Cell Seeding
- 02:37Endothelial Cell Seeding and Long-term Perfusion
- 04:38Immunofluorescent Staining and Imaging Calcium Concentration or Nitric Oxide Production
- 06:39Data Analysis
- 07:35Results: Endothelial Cell Culture Under Shear Stress
- 08:39Conclusion
Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.
