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Generation af ESC-afledt Mouse Airway epitelceller Brug decellulariserede Lung Stilladser
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Chapitres
- 00:05Titre
- 00:47Scaffold Preparation
- 03:57Thick Section Generation
- 07:17Decontamination of Scaffold Sections and Recellularization Setup
- 09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
- 11:52Conclusion
Denne protokol effektivt dirigerer muse embryonale stamceller afledt endelig endoderm til modne luftvejs-epitelceller. Denne differentiering teknik anvender 3-dimensionelle decellulariseret lunge stilladser til direkte lunge afstamning specifikation, i en defineret, serumfrit indstilling kultur.
Tags
Decellularized Lung ScaffoldsEmbryonic Stem Cell DifferentiationAirway Epithelial Cells3D CultureLung DevelopmentCell Matrix ContractionsGrowth Factor SignalingSerum-free SettingRobust Airway Epithelial DifferentiationLimited Endoderm Lineage ContaminationRat LungsPerfusionTracheal CannulationDecellularization Solution
