Journal
/
/
皮膚外科神経切除:皮膚病のマウスモデルにおける神経のための要件をテストする方法
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
DOI:

08:01 min

June 26, 2016

, ,

Chapitres

  • 00:05Titre
  • 00:43Collecting Skin Biopsies
  • 02:49Surgical Denervation
  • 05:36Behavioral Test of Denervation
  • 06:22Results: Touch Dome Cells and Merkel Cells Require Innervation
  • 07:20Conclusion

Summary

Traduction automatique

This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.

Vidéos Connexes

Read Article