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Vurdering av Myofilament Ca<sup> 2+</sup> Følsomhet underliggende hjerte Eksitasjon-kontraksjon Coupling
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Journal JoVE Biologie
Assessment of Myofilament Ca2+ Sensitivity Underlying Cardiac Excitation-contraction Coupling

Vurdering av Myofilament Ca<sup> 2+</sup> Følsomhet underliggende hjerte Eksitasjon-kontraksjon Coupling

DOI:
10.3791/54057-v

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08:29 min

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August 01, 2016

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, , , , , , ,
1Department of Physiology & Biomedical Sciences, Ischemic/hypoxic Disease Institute,Seoul National University College of Medicine, 2Yan Bian University Hospital, 3Institute of Cardiovascular Sciences,University of Manchester

Chapitres

  • 00:05Titre
  • 00:49Preparation
  • 01:58Isolation of Left Ventricular Myocytes
  • 04:22Simultaneous Recordings of Ca2+ Transients and Contraction
  • 06:33Analysis of the Myofilament Ca2+ Sensitivity
  • 07:17Conclusion

Summary

Traduction automatique

Traduction automatique

This paper describes a protocol that assesses the changes of myofilament Ca2+ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca2+ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts.

Tags

Myofilament Ca2+ SensitivityCardiac Excitation-contraction CouplingHeart ContractilityHeart FailureLangendorff Perfusion SystemCollagenaseIsolation SolutionSD RatPentobarbital SodiumLeft Ventricular (LV) Free WallMyocytes

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