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Die Förderung der 3-D-Aggregation von FACS Purified Thymusepithelzellen mit EAK 16-II / EAKIIH6 Selbstaufbau Hydrogels
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
DOI:

08:02 min

June 27, 2016

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Chapitres

  • 00:05Titre
  • 00:51Thymus Digestion
  • 03:50Thymic Epithelial Cell (TEC) Enrichment
  • 04:58TEC/EAK Aggregate Generation
  • 06:52Results: Representative TEC Enrichment and Aggregation
  • 07:26Conclusion

Summary

Traduction automatique

This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.

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