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Genome Redigerer i<em> Astyanax mexicanus</em> Ved hjelp av transkripsjon Activator-lignende Effector nukleaser (Talens)
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Journal JoVE Biologie
Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)

Genome Redigerer i<em> Astyanax mexicanus</em> Ved hjelp av transkripsjon Activator-lignende Effector nukleaser (Talens)

DOI:
10.3791/54113-v

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07:42 min

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June 20, 2016

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, ,
1Genetics, Development and Cell Biology,Iowa State University, 2Department of Biological Sciences,University of Cincinnati, 3Department of Biology,University of Maryland

Chapitres

  • 00:05Titre
  • 00:34mRNA Transcription of Transcription Activator-like Effector Nucleases
  • 02:18Inject Astyanax mexicanus Embryos with TALEN mRNA
  • 04:06Phenotype Founder Fish and Evaluate TALEN Efficiency
  • 05:27Results: Germline Transmission of TALEN-induced oca2 Mutations in A. mexicanus
  • 06:47Conclusion

Summary

Traduction automatique

Traduction automatique

Gene-målretting mutagenese er nå mulig i et bredt spekter av organismer ved bruk av genomet redigering teknikker. Her viser vi en protokoll for målrettet genet mutagenese ved hjelp av transkripsjon aktivator som effektor nukleaser (Talens) i Astyanax mexicanus, en fiskeart som inkluderer overflaten fisk og cavefish.

Tags

Keywords: Genome EditingAstyanax MexicanusTranscription Activator-like Effector Nucleases (TALENs)Evolutionary BiologyCave TraitsMRNA ProductionMicroinjectionSurface Fish Eggs

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