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1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center,Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine,Yale University School of Medicine, 3Department of Molecular and Cellular Biology,Baylor College of Medicine
Chapitres
- 00:05Titre
- 01:12Mouse Embryo Yolk Sac (YS) or Aorta-gonad-mesonephros (AGM) Dissection
- 03:29Primary YS/AGM Tissue Digestion
- 04:37Nucleic Acid Dye- and Fluorescently Conjugated Antibody-labeling
- 06:19Results: Representative Flow Cytometric Identification of Hemogenic Endothelial Cells (EC), Hematopoetic Stem and Progenitor Cells (HSPC), and Non-hemogenic EC
- 07:43Conclusion
Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.
