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Immunologie et infection
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Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi
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Immunologie et infection
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Journal JoVE Immunologie et infection
Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

DOI:
10.3791/54162-v

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12:33 min

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July 06, 2016

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, , ,
1Department of Microbiology and Immunology,Midwestern University

Chapitres

  • 00:05Titre
  • 01:16HSV Strains and Handling
  • 04:59Polymicrobial Biofilm Assay
  • 07:27Imaging Studies
  • 09:56Results: Microbial Adherence to HSV Infected Cells
  • 11:13Conclusion

Summary

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A method is described herein for the determination of inter-Kingdom association and competition (bacterial and fungal) for adherence to virus-infected HeLa cell monolayers. This protocol can be extended to multiple combinations of prokaryotes, eukaryotes, and viruses.

Tags

BiofilmViral InfectionOpportunistic PathogensStaphylococcus AureusCandida AlbicansHSVCMVAdenovirusHeLa CellsC. AlbicansGerm TubeYeast FormAdherenceAttachmentMicrobiomeMultiplicity Of InfectionReporter VirusParaformaldehyde GlutaraldehydeDMEMTrypsinHBSSOD 600

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