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Osservazione e quantificazione dei telomeri e sequenze ripetitive Usando fluorescenza<em> In Situ</em> Ibridazione (FISH) con sonde PNA in<em> Caenorhabditis elegans</em
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Journal JoVE Biologie
Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans
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Osservazione e quantificazione dei telomeri e sequenze ripetitive Usando fluorescenza<em> In Situ</em> Ibridazione (FISH) con sonde PNA in<em> Caenorhabditis elegans</em

DOI:
10.3791/54224-v

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10:01 min

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August 04, 2016

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,
1Institute of Molecular Biology and Genetics (IMBG),Seoul National University, 2Department of Biological Sciences,Seoul National University, 3Department of Biophysics and Chemical Biology,Seoul National University

Chapitres

  • 00:05Titre
  • 00:37Fixation of C. elegans
  • 03:00Permeabilization
  • 04:59Hybridization
  • 06:42Washing and Immunofluorescent Staining
  • 08:41Results: Telomere and TALT1 FISH in the Embryos
  • 09:33Conclusion

Summary

Traduction automatique

Traduction automatique

We report a concise procedure of fluorescence in situ hybridization (FISH) in the gonad and embryos of Caenorhabditis elegans for observing and quantifying repetitive sequences. We successfully observed and quantified two different repetitive sequences, telomere repeats and template of alternative lengthening of telomeres (TALT).

Tags

Keywords: TelomereFluorescence In Situ HybridizationFISHPNA ProbesCaenorhabditis ElegansWormEggFixationBleachingM9 BufferPolylysine Coated SlidesRepetitive SequencesCellular SenescenceTumor RegenesisAlternative Lengthening Of Telomeres

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