/
/
Teknikker til Udviklingen Robust pentose-gæring Gær til Biokonversion af lignocellulose til ethanol
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:40Enrichment of Robust Derivatives during Serial Transfer on AFEX CSH
- 03:42Isolation of Single Cell Tolerant Derivatives after Enrichment on AFEX CSH
- 04:58Performance Evaluation of AFEX CSH Tolerant Derivatives Compared to Parent
- 07:15Applying Continuous Culture to Select for Ethanol-challenged Xylose Utilization
- 09:43Isolation of Single-cell Colonies That Utilize Xylose in PSGHL When Ethanol Is Present
- 11:20Results: Derivatives of S. stipitis Parent Strain Have Improved Efficiency in Converting Hydrolyzates to Ethanol
- 12:54Conclusion
Adaptive evolution og isolation teknikker er beskrevet og demonstreret at give derivater af Scheffersomyces stipitis stammen NRRL Y-7124, der er i stand til hurtigt at forbruge hexose og pentose blandede sukker i enzym forsukret undetoxified hydrolysater og at akkumulere over 40 g / l ethanol.
